癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (5): 404-406.doi: 10.3969/j.issn.1004-616x.2006.05.021

• 技术与方法 • 上一篇    下一篇

Mini Zoom IPG Runner系统双向电泳分析血浆蛋白实验条件优化

周海涛1,2/朱　 红3/龚英浩4/胡金森1/高　宙1/贾少微1/黎明涛2   

  1. 1.北京大学深圳医院核医学科，广东　深圳　518036；2.中山大学基础医学院蛋白质组学实验室，广东　广州　510182；3.中国医科大学第一临床学院皮肤科，辽宁　沈阳　110001，4.北京大学深圳医院门诊操作治疗区，广东　深圳　518036
  • 收稿日期:2006-04-30 修回日期:2006-06-12 出版日期:2006-09-30 发布日期:2006-09-30
  • 通讯作者: 周海涛

Optimizing Protocol of Two-Dim-ensional Electrophoresis by Mini Zoom IPG Runner System for the Analysis of Plasma Proteins

ZHOU Hai-Tao1,2, ZHU Hong3, GONG Ying-Hao4, HU Jin-Sen1, GAO Zhou1, JIA Shao-Wei1, LI Ming-Tao2   

  1. 1.Department of Nuclear Medicine，Peking University Shenzhen Hospital, Shenzhen 518036; 2.Proteomics Laboratory, Zhongshan Medical College, Sun Yat-sen University, Guanzhou 510060; 3.Department of Dermatology,No.1 Hospital of China Medical University, Shenyang 110001; 4.Outpatient Treatment Area，Peking University Shenzhen Hospital, Shenzhen 518036,Guangdong,China
  • Received:2006-04-30 Revised:2006-06-12 Online:2006-09-30 Published:2006-09-30
  • Contact: ZHOU Hai-Tao1

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摘要： 背景与目的： 优化Mini Zoom IPG Runner系统双向电泳条件。 材料与方法： 使用Invitrogen公司与Mini Zoom IPG Runner系统配套的商品化预制胶(一向和二向)和溶液体系对血浆蛋白进行双向电泳，采用不同的聚焦条件和样品预处理，观察其对实验结果的影响。 结果： 提高聚焦电压增强聚焦及脱盐处理可获得比推荐条件更好的双向电泳图谱。 结论： 提高聚焦电压增强聚焦及脱盐处理可改善Mini Zoom IPG Runner系统分析血浆样品的双向电泳结果。

关键词: 血浆蛋白质组, Mini Zoom IPG Runner系统, 双向电泳

Abstract: BACKGROUND ＆ AIM: Optimizing the protocol of two-dimensional electrophoresis by Mini Zoom IPG Runner System to analyze plasma proteins. MATERIAL AND MATHODS: Using the immobile IPG strips, ZOOMTM Gels for 2D Electrophoresis and buffer solution system purchased from Invitrogen,two dimensional electrophoresis of plasma proteins was performed.The effect of changing the condition of isoelectric focusing and pretreating the samples,on the two dimensional profiles was analyzed so as to optimize the protocol of the system. RESULTS: Compared with the recommended condition, the profiles were improved by enhancing the voltage of isoelectric focusing, extending the time and desalinizing the samples. CONCLUSION: The protocol of two-dimensional electrophoresis by Mini Zoom IPG Runner System for the analysis of plasma proteins should be optimized by increasing the voltage of isoelectric focusing, extending the time and desalinizing samples.

Key words: plasma proteins, two dimensional electrophoresis, Mini Zoom IPG Runner system

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